TY - JOUR
T1 - Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish
AU - Liu, Chunsheng
AU - Yu, Liqin
AU - Deng, Jun
AU - Lam, Paul K.S.
AU - Wu, Rudolf S.S.
AU - Zhou, Bingsheng
N1 - Funding Information:
This study was supported by the National Nature Science Foundation of China (20890113 and 20877094) and State Key Laboratory of Freshwater Ecology and Biotechnology (2008FBZ10).
PY - 2009/6/28
Y1 - 2009/6/28
N2 - Fluorotelomer alcohols (FTOHs) have shown estrogenic activity in vitro and in vivo, but the mechanism of this activity is not known. In this study, 18-week-old zebrafish (Danio rerio) were exposed to 0, 0.03, 0.3 and 3.0 mg/l 1H,1H,2H,2H-perfluorooctan-1-ol (6:2 FTOH) for 7 days, and the effects on plasma sex hormone levels were measured followed by use of real-time PCR to examine selected gene expression in hypothalamic-pituitary-gonadal (HPG) axis and liver. Exposure to 6:2 FTOH significantly increased plasma estradiol (E2) and testosterone (T) levels in both males and females. Furthermore, the ratio of T/E2 was reduced in females while increased in males. In females, the increase of E2 was accompanied by up-regulated hepatic estrogenic receptor α (ERα) and vitellogenin (VTG1 and VTG3) expression. In males, the elevation of the T level is consistent with the up-regulation of cytochrome P450 c17α-hydroxylase, 17, 20-lase (CYP17) and the down-regulation of cytochrome P450 aromatase A (CYP19A). The present study demonstrated that waterborne exposure to 6:2 FTOH alter plasma sex hormone levels and the ratio of T/E2, as well as the transcriptional profiles of some genes in the HPG axis and liver. The results suggested that FTOHs may disturb fish reproduction through endocrine disrupted activity.
AB - Fluorotelomer alcohols (FTOHs) have shown estrogenic activity in vitro and in vivo, but the mechanism of this activity is not known. In this study, 18-week-old zebrafish (Danio rerio) were exposed to 0, 0.03, 0.3 and 3.0 mg/l 1H,1H,2H,2H-perfluorooctan-1-ol (6:2 FTOH) for 7 days, and the effects on plasma sex hormone levels were measured followed by use of real-time PCR to examine selected gene expression in hypothalamic-pituitary-gonadal (HPG) axis and liver. Exposure to 6:2 FTOH significantly increased plasma estradiol (E2) and testosterone (T) levels in both males and females. Furthermore, the ratio of T/E2 was reduced in females while increased in males. In females, the increase of E2 was accompanied by up-regulated hepatic estrogenic receptor α (ERα) and vitellogenin (VTG1 and VTG3) expression. In males, the elevation of the T level is consistent with the up-regulation of cytochrome P450 c17α-hydroxylase, 17, 20-lase (CYP17) and the down-regulation of cytochrome P450 aromatase A (CYP19A). The present study demonstrated that waterborne exposure to 6:2 FTOH alter plasma sex hormone levels and the ratio of T/E2, as well as the transcriptional profiles of some genes in the HPG axis and liver. The results suggested that FTOHs may disturb fish reproduction through endocrine disrupted activity.
KW - Endocrine disruption
KW - Fluorotelomer alcohols
KW - Gene transcription
KW - Hypothalamic-pituitary-gonadal (HPG) axis
KW - Sex hormone
UR - https://www.scopus.com/pages/publications/67349250916
U2 - 10.1016/j.aquatox.2009.04.005
DO - 10.1016/j.aquatox.2009.04.005
M3 - Article
C2 - 19443055
AN - SCOPUS:67349250916
SN - 0166-445X
VL - 93
SP - 131
EP - 137
JO - Aquatic Toxicology
JF - Aquatic Toxicology
IS - 2-3
ER -