Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

L. H. Xu; P. K.S. Lam; J. P. Chen; J. M. Xu; B. S.F. Wong; Y. Y. Zhang; R. S.S. Wu; K. I. Harada

doi:10.1016/S0045-6535(99)00389-6

Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

L. H. Xu, P. K.S. Lam, J. P. Chen, J. M. Xu, B. S.F. Wong, Y. Y. Zhang, R. S.S. Wu, K. I. Harada

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive ³²P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Chemosphere
Volume	41
Issue number	1-2
DOIs	https://doi.org/10.1016/S0045-6535(99)00389-6
Publication status	Published - Jul 2000
Externally published	Yes

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@article{e223fd67d2d1411ebc64aa97ae23a35e,

title = "Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China",

abstract = "Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.",

author = "Xu, {L. H.} and Lam, {P. K.S.} and Chen, {J. P.} and Xu, {J. M.} and Wong, {B. S.F.} and Zhang, {Y. Y.} and Wu, {R. S.S.} and Harada, {K. I.}",

note = "Funding Information: The authors would like to thank Prof. Xie Ping and his research team for collecting water samples from Donghu Lake. We thank also Prof. Yu Jialu for the identification of cyanobacteria. This work was funded by the Natural Science Foundation of China (Project No.: 39400024) and the Hong Kong Research Grants Council (No.: 9040278).",

year = "2000",

month = jul,

doi = "10.1016/S0045-6535(99)00389-6",

language = "English",

volume = "41",

pages = "53--58",

number = "1-2",

}

TY - JOUR

T1 - Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

AU - Xu, L. H.

AU - Lam, P. K.S.

AU - Chen, J. P.

AU - Xu, J. M.

AU - Wong, B. S.F.

AU - Zhang, Y. Y.

AU - Wu, R. S.S.

AU - Harada, K. I.

N1 - Funding Information: The authors would like to thank Prof. Xie Ping and his research team for collecting water samples from Donghu Lake. We thank also Prof. Yu Jialu for the identification of cyanobacteria. This work was funded by the Natural Science Foundation of China (Project No.: 39400024) and the Hong Kong Research Grants Council (No.: 9040278).

PY - 2000/7

Y1 - 2000/7

N2 - Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.

AB - Seasonal variations in the level of total microcystins in water samples collected from Donghu Lake and a fish pond in Wuhan, China, were studied between March 1995 and February 1996 using a protein phosphatase inhibition assay involving a radioactive 32P-labelled substrate. The assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. Results of the survey indicated the presence of microcystins in the water samples, and the concentration of microcystins appeared to be related to the degree of eutrophication and water temperature. There is also a correlative relationship between the quantity of microcystins and the abundance of microcystin-producing cyanobacteria (Anabaena and Oscillatoria) in the water bodies over a year cycle. In the present study, the positive detection of microcystins in water bodies having no signs of algal bloom warns of considerable potential threat of these waters to public health. (C) 2000 Elsevier Science Ltd.

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U2 - 10.1016/S0045-6535(99)00389-6

DO - 10.1016/S0045-6535(99)00389-6

M3 - Article

C2 - 10819179

AN - SCOPUS:0342859372

SN - 0045-6535

VL - 41

SP - 53

EP - 58

JO - Chemosphere

JF - Chemosphere

IS - 1-2

ER -

Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China

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