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Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells

  • Bowie P. Lam
  • , Ka Chi Sarah Cheung
  • , Yun Wah Lam
  • , Stella W. Pang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu mathrm{m} narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu mathrm{m} wide trenches after being stimulated by transforming growth factor beta-1 (text{TGF}-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.

Original languageEnglish
Title of host publication33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Pages412-415
Number of pages4
ISBN (Electronic)9781728135809
DOIs
Publication statusPublished - Jan 2020
Externally publishedYes
Event33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada
Duration: 18 Jan 202022 Jan 2020

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2020-January
ISSN (Print)1084-6999

Conference

Conference33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Country/TerritoryCanada
CityVancouver
Period18/01/2022/01/20

Keywords

  • 3D Scaffold Platform
  • Biomimetic
  • Cell Migration
  • Drug Screening
  • NP460 Cells

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