TY - JOUR
T1 - Temporal changes in Sphingomonas and Mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (PAHs)
AU - Guo, Chuling
AU - Ke, Lin
AU - Dang, Zhi
AU - Tam, Nora Fungyee
N1 - Funding Information:
The research work described in this paper was supported by grants from the Strategic Research Grant of the City University of Hong Kong (Project No.: 7008036), the Natural Research Fund of Guangdong Province , Guangdong (Project No.: 9351064101000001) and the Fundamental Research Funds for the Central Universities of China (Project No.: 2009ZM0034).
PY - 2011/1
Y1 - 2011/1
N2 - The change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (PAH)-degrading bacterial genera, Sphingomonas and Mycobacterium, due to contamination in the environment is not very well known. A combination of PCR-DGGE with specific primers and a cultivation-dependent microbiological method was used to detect different populations of Sphingomonas and Mycobacterium in mangrove sediments. The structure of the entire bacterial community (including Sphingomonas) did not show a shift due to environmental contamination, whereas the diversity of Mycobacterium populations in mangrove sediments with higher PAH contamination increased from exposure between Day 0 and Day 30. The isolated Mycobacterium strains migrated to the same position as the major bands of the bacterial communities in Mycobacterium-specific DGGE. A dioxygenase gene system, nidA, which is commonly found in PAH-degrading Mycobacterium strains, was also detected in the more highly contaminated sediment slurries. The present study revealed that Mycobacterium species were the dominant PAH-degraders and played an important role in degrading PAHs in contaminated mangrove sediments.
AB - The change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (PAH)-degrading bacterial genera, Sphingomonas and Mycobacterium, due to contamination in the environment is not very well known. A combination of PCR-DGGE with specific primers and a cultivation-dependent microbiological method was used to detect different populations of Sphingomonas and Mycobacterium in mangrove sediments. The structure of the entire bacterial community (including Sphingomonas) did not show a shift due to environmental contamination, whereas the diversity of Mycobacterium populations in mangrove sediments with higher PAH contamination increased from exposure between Day 0 and Day 30. The isolated Mycobacterium strains migrated to the same position as the major bands of the bacterial communities in Mycobacterium-specific DGGE. A dioxygenase gene system, nidA, which is commonly found in PAH-degrading Mycobacterium strains, was also detected in the more highly contaminated sediment slurries. The present study revealed that Mycobacterium species were the dominant PAH-degraders and played an important role in degrading PAHs in contaminated mangrove sediments.
KW - Mangrove sediment
KW - Microbial community structure
KW - Mycobacterium
KW - PAH-degrading bacteria
KW - PAHs
KW - Sphingomonas
UR - http://www.scopus.com/inward/record.url?scp=78751615744&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2010.08.022
DO - 10.1016/j.marpolbul.2010.08.022
M3 - Article
C2 - 20926106
AN - SCOPUS:78751615744
SN - 0025-326X
VL - 62
SP - 133
EP - 139
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 1
ER -