Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers: Via immuno-capture LI-MS

Toby Siu Chung Tam; Yu Hong Cheng; Chun Nam Lok; Ho Yu Au-Yeung; Wen Xiu Ni; Xiao Long Wei; Kwan Ming Ng

doi:10.1039/d0an01121e

Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers: Via immuno-capture LI-MS

Toby Siu Chung Tam
, Yu Hong Cheng
, Chun Nam Lok
, Ho Yu Au-Yeung
, Wen Xiu Ni
, Xiao Long Wei
, Kwan Ming Ng

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.

Original language	English
Pages (from-to)	6237-6242
Number of pages	6
Journal	Analyst
Volume	145
Issue number	19
DOIs	https://doi.org/10.1039/d0an01121e
Publication status	Published - 7 Oct 2020
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.1039/d0an01121e

Cite this

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title = "Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers: Via immuno-capture LI-MS",

abstract = "HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.",

author = "Tam, \{Toby Siu Chung\} and Cheng, \{Yu Hong\} and Lok, \{Chun Nam\} and Au-Yeung, \{Ho Yu\} and Ni, \{Wen Xiu\} and Wei, \{Xiao Long\} and Ng, \{Kwan Ming\}",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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doi = "10.1039/d0an01121e",

language = "English",

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TY - JOUR

T1 - Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers

T2 - Via immuno-capture LI-MS

AU - Tam, Toby Siu Chung

AU - Cheng, Yu Hong

AU - Lok, Chun Nam

AU - Au-Yeung, Ho Yu

AU - Ni, Wen Xiu

AU - Wei, Xiao Long

AU - Ng, Kwan Ming

PY - 2020/10/7

Y1 - 2020/10/7

N2 - HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.

AB - HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.

UR - https://www.scopus.com/pages/publications/85091808523

U2 - 10.1039/d0an01121e

DO - 10.1039/d0an01121e

M3 - Article

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AN - SCOPUS:85091808523

SN - 0003-2654

VL - 145

SP - 6237

EP - 6242

JO - Analyst

JF - Analyst

IS - 19

ER -

Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers: Via immuno-capture LI-MS

Abstract

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