TY - JOUR
T1 - Study on the cytotoxicity of microcystin-LR on cultured cells
AU - Chong, M. W.K.
AU - Gu, K. D.
AU - Lam, P. K.S.
AU - Yang, M.
AU - Fong, W. F.
N1 - Funding Information:
This work was sponsored by the Hong Kong Research Grants Council (No.: CityU1057/97M). We thank Mr. P.P. Yung for his technical assistance.
PY - 2000/7
Y1 - 2000/7
N2 - The toxicity of purified blue-green algal toxin, microcystin-LR, on permanent cell lines KB, NIH/3T3, H-4-II-E, HeLa, Vero, Hep G2, Caco-2 and HL-60 was studied. Assessment of cell viability using colorimetric 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays indicated that purified microcystin-LR induced toxic effect on KB and H-4-II-E cell lines after 96 h incubation at toxin concentrations greater than 18.75 μg/ml. KB cell line was selected for further study when reproducibility, consistency and sensitivity were considered. Significant amounts of lactate dehydrogenase (LDH) were released from KB cells when incubation durations were 72 and 96 h with toxin concentrations of 18.75 μg/ml and higher. Although previous studies suggested that microcystin-LR had no cytotoxic effect on permanent cell lines, LDH release assay performed on KB cells indicated that exposure to microcystin-LR could result in damage to the cell membrane. (C) 2000 Elsevier Science Ltd.
AB - The toxicity of purified blue-green algal toxin, microcystin-LR, on permanent cell lines KB, NIH/3T3, H-4-II-E, HeLa, Vero, Hep G2, Caco-2 and HL-60 was studied. Assessment of cell viability using colorimetric 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays indicated that purified microcystin-LR induced toxic effect on KB and H-4-II-E cell lines after 96 h incubation at toxin concentrations greater than 18.75 μg/ml. KB cell line was selected for further study when reproducibility, consistency and sensitivity were considered. Significant amounts of lactate dehydrogenase (LDH) were released from KB cells when incubation durations were 72 and 96 h with toxin concentrations of 18.75 μg/ml and higher. Although previous studies suggested that microcystin-LR had no cytotoxic effect on permanent cell lines, LDH release assay performed on KB cells indicated that exposure to microcystin-LR could result in damage to the cell membrane. (C) 2000 Elsevier Science Ltd.
UR - http://www.scopus.com/inward/record.url?scp=0034006893&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(99)00402-6
DO - 10.1016/S0045-6535(99)00402-6
M3 - Article
C2 - 10819192
AN - SCOPUS:0034006893
SN - 0045-6535
VL - 41
SP - 143
EP - 147
JO - Chemosphere
JF - Chemosphere
IS - 1-2
ER -
