TY - JOUR
T1 - Study of metabolites from the degradation of polycyclic aromatic hydrocarbons (PAHs) by bacterial consortium enriched from mangrove sediments
AU - Luan, T. G.
AU - Yu, Keith S.H.
AU - Zhong, Y.
AU - Zhou, H. W.
AU - Lan, C. Y.
AU - Tam, Nora F.Y.
N1 - Funding Information:
The work described in this paper was financially supported by a grant from CityU (Project No. 7001690), and National Natural Foundation of China (NSFC, No. 20307012).
PY - 2006/12
Y1 - 2006/12
N2 - The PAH metabolites produced during degradation of fluorene, phenanthrene and pyrene by a bacterial consortium enriched from mangrove sediments were analyzed using the on-fiber silylation solid-phase microextraction (SPME) combining with gas chromatography-mass spectrometry (GC-MS) method. Seventeen metabolites at trace levels were identified in different PAH degradation cultures based on the full scan mass spectra. In fluorene degradation cultures, 1-, 2-, 3- and 9-hydroxyfluorene, fluorenone, and phthalic acid were detected. In phenanthrene and pyrene degradation cultures, various common metabolites such as phenanthrene and pyrene dihydrodiols, mono-hydroxy phenanthrene, dihydroxy pyrene, lactone and 4-hydroxyphenanthrene, methyl ester, and phthalic acid were found. The detection of various common and novel metabolites demonstrates that SPME combining with GC-MS is a quick and convenient method for identification as well as monitoring the real time changes of metabolite concentrations throughout the degradation processes. The knowledge of PAH metabolic pathways and kinetics within indigenous bacterial consortium enriched from mangrove sediments contributes to enhance the bioremediation efficiency of PAH in real environment.
AB - The PAH metabolites produced during degradation of fluorene, phenanthrene and pyrene by a bacterial consortium enriched from mangrove sediments were analyzed using the on-fiber silylation solid-phase microextraction (SPME) combining with gas chromatography-mass spectrometry (GC-MS) method. Seventeen metabolites at trace levels were identified in different PAH degradation cultures based on the full scan mass spectra. In fluorene degradation cultures, 1-, 2-, 3- and 9-hydroxyfluorene, fluorenone, and phthalic acid were detected. In phenanthrene and pyrene degradation cultures, various common metabolites such as phenanthrene and pyrene dihydrodiols, mono-hydroxy phenanthrene, dihydroxy pyrene, lactone and 4-hydroxyphenanthrene, methyl ester, and phthalic acid were found. The detection of various common and novel metabolites demonstrates that SPME combining with GC-MS is a quick and convenient method for identification as well as monitoring the real time changes of metabolite concentrations throughout the degradation processes. The knowledge of PAH metabolic pathways and kinetics within indigenous bacterial consortium enriched from mangrove sediments contributes to enhance the bioremediation efficiency of PAH in real environment.
KW - Biodegradation
KW - Fluorene
KW - Gas chromatography-mass spectrometry (GC-MS)
KW - Phenanthrene
KW - Pyrene
KW - Solid phase microextraction
UR - http://www.scopus.com/inward/record.url?scp=33751006738&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2006.05.013
DO - 10.1016/j.chemosphere.2006.05.013
M3 - Article
C2 - 16806399
AN - SCOPUS:33751006738
SN - 0045-6535
VL - 65
SP - 2289
EP - 2296
JO - Chemosphere
JF - Chemosphere
IS - 11
ER -