TY - JOUR
T1 - Pyrene-induced changes of glutathione-S-transferase activities in different microalgal species
AU - Lei, An Ping
AU - Wong, Yuk Shan
AU - Tam, Nora Fung yee
N1 - Funding Information:
The work described in this paper was supported by a grant from the Research Grant Council of the HKSAR (project number: 9040661) and this financial support is acknowledged. We would like to thank all the group members of Prof. Nora Tam and Prof. Yuk-shan Wong for their co-operation. Many thanks also go to the anonymous reviewers for their constructive suggestions.
PY - 2003/1
Y1 - 2003/1
N2 - The glutathione-S-transferase (GST, EC 2.5.1.18) activities in different freshwater microalgal species, namely, Chlorella vulgaris, Scenedesmus quadricauda, Scenedesmus platydiscus and Selenastrum capricornutum under the control condition (without pyrene addition) and at different pyrene concentrations were compared. During 7-days incubation under the control condition (without pyrene addition), all microalgal species exhibited measurable GST activities but the activities varied significantly among species and the difference could be more than 100-fold. The addition of pyrene at concentrations ranged from 0.1 to 1.0 mg l-1 to microalgal cultures led to changes in GST activities but the patterns of changes varied from species to species. Among the four species, remarkably decreases in GST activities were found in S. quadricauda, a species most sensitive to pyrene toxicity, at high pyrene concentrations. On the contrary, GST activities in S. platydiscus and Se. capricornutum increased significantly as pyrene concentrations increased. These two species were found to be more resistant to pyrene and had higher efficiencies in metabolising pyrene than other species. C. vulgaris did not show any significant change in their GST activities with the addition of pyrene, and pyrene was not metabolised by this species. These results suggest that pyrene-induced changes of GST activities in microalgae might be related to their resistance and their ability to metabolise pyrene. In general, the pyrene-induced changes of GST activities were higher at 4-days than at 1- and 7-days incubation in all microalgae.
AB - The glutathione-S-transferase (GST, EC 2.5.1.18) activities in different freshwater microalgal species, namely, Chlorella vulgaris, Scenedesmus quadricauda, Scenedesmus platydiscus and Selenastrum capricornutum under the control condition (without pyrene addition) and at different pyrene concentrations were compared. During 7-days incubation under the control condition (without pyrene addition), all microalgal species exhibited measurable GST activities but the activities varied significantly among species and the difference could be more than 100-fold. The addition of pyrene at concentrations ranged from 0.1 to 1.0 mg l-1 to microalgal cultures led to changes in GST activities but the patterns of changes varied from species to species. Among the four species, remarkably decreases in GST activities were found in S. quadricauda, a species most sensitive to pyrene toxicity, at high pyrene concentrations. On the contrary, GST activities in S. platydiscus and Se. capricornutum increased significantly as pyrene concentrations increased. These two species were found to be more resistant to pyrene and had higher efficiencies in metabolising pyrene than other species. C. vulgaris did not show any significant change in their GST activities with the addition of pyrene, and pyrene was not metabolised by this species. These results suggest that pyrene-induced changes of GST activities in microalgae might be related to their resistance and their ability to metabolise pyrene. In general, the pyrene-induced changes of GST activities were higher at 4-days than at 1- and 7-days incubation in all microalgae.
KW - Bioremediation
KW - Chlorella
KW - Glutathione-S-transferase
KW - Polycyclic aromatic hydrocarbons
KW - Scenedesmus
KW - Selenastrum
UR - http://www.scopus.com/inward/record.url?scp=0037230917&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(02)00499-X
DO - 10.1016/S0045-6535(02)00499-X
M3 - Article
C2 - 12656248
AN - SCOPUS:0037230917
SN - 0045-6535
VL - 50
SP - 293
EP - 301
JO - Chemosphere
JF - Chemosphere
IS - 3
ER -