TY - JOUR
T1 - Production of metabolites in the biodegradation of phenanthrene, fluoranthene and pyrene by the mixed culture of Mycobacterium sp. and Sphingomonas sp
AU - Zhong, Yin
AU - Luan, Tiangang
AU - Lin, Li
AU - Liu, Hong
AU - Tam, Nora F.Y.
N1 - Funding Information:
We thank Y.S. Wong, Lilian Vrijmoed, Paul Shin and S.G. Cheung for their advice and assistance. The financial support from National Natural Science Foundation of China ( NSFC, No. 20977116 ), Natural Scientific Foundation of Guangdong Province ( No. 9451064004002394 ) and Program for New Century Excellent Talents in University, as well as from the Strategic Research Grants from City University of Hong Kong (Project No. 7008036 ) are gratefully acknowledged. The Administrative Bureau of Guangdong Neilingding Futian National Nature Reserve is also acknowledged.
PY - 2011/2
Y1 - 2011/2
N2 - The effects of the mixed culture of Mycobacterium sp. strain A1-PYR and Sphingomonas sp. strain PheB4 on the degradation characteristics of single polycyclic aromatic hydrocarbon were investigated. In the mixed bacterial culture, phenanthrene, fluoranthene and pyrene were degraded by 100% at Day 3, 71.2% and 50% at Day 7, respectively. Compared to their respective pure cultures, the degradation of phenanthrene and fluoranthene decreased, but that of pyrene increased significantly. Based on GC-MS analysis, eight and six new metabolites were produced from the biodegradation of phenanthrene and fluoranthene, respectively, while only two new metabolites were formed from pyrene. To our knowledge, this is the first report that the mixed bacterial culture could increase the diversity of metabolites from PAH, but the diverse metabolite pattern was not necessarily beneficial to the degradation of the recalcitrant PAH. The enhancement on pyrene degradation was possibly attributed to the rapid growth of strain PheB4.
AB - The effects of the mixed culture of Mycobacterium sp. strain A1-PYR and Sphingomonas sp. strain PheB4 on the degradation characteristics of single polycyclic aromatic hydrocarbon were investigated. In the mixed bacterial culture, phenanthrene, fluoranthene and pyrene were degraded by 100% at Day 3, 71.2% and 50% at Day 7, respectively. Compared to their respective pure cultures, the degradation of phenanthrene and fluoranthene decreased, but that of pyrene increased significantly. Based on GC-MS analysis, eight and six new metabolites were produced from the biodegradation of phenanthrene and fluoranthene, respectively, while only two new metabolites were formed from pyrene. To our knowledge, this is the first report that the mixed bacterial culture could increase the diversity of metabolites from PAH, but the diverse metabolite pattern was not necessarily beneficial to the degradation of the recalcitrant PAH. The enhancement on pyrene degradation was possibly attributed to the rapid growth of strain PheB4.
KW - Biodegradation
KW - Metabolite
KW - Mixed bacterial culture
KW - PAH
KW - Pathway
UR - http://www.scopus.com/inward/record.url?scp=78650833792&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.09.113
DO - 10.1016/j.biortech.2010.09.113
M3 - Article
C2 - 21036605
AN - SCOPUS:78650833792
SN - 0960-8524
VL - 102
SP - 2965
EP - 2972
JO - Bioresource Technology
JF - Bioresource Technology
IS - 3
ER -