Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer

Lui Ng, Timothy Ming Hun Wan, Colin Siu Chi Lam, Ariel Ka Man Chow, Sunny Kit Man Wong, Johnny Hon Wai Man, Hung Sing Li, Nathan Shiu Man Cheng, Ryan Chung Hei Pak, Alvin Ho Kwan Cheung, Thomas Chung Cheung Yau, Oswens Siu Hung Lo, Dominic Chi Chung Foo, Jensen Tung Chung Poon, Ronnie Tung Ping Poon, Roberta Wen Chi Pang, Wai Lun Law

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Background: The overall prognosis of colorectal cancer (CRC) patients is unsatisfactory due to cancer metastasis after operation. This study aims to investigate the clinical significance of plasma osteopontin (OPN) levels as minimally invasive, predictive, and surrogate biomarkers for prognosis of CRC patients. Methods: This randomized study design consists of pre-operative and post-operative plasma samples from a total of 79 patients. We determined plasma levels of OPN by ELISA and examined their correlation with the clinicopathological parameters of CRC patients. The effects of endogenous and exogenous OPN on CRC metastasis were investigated by examination of the effect on regulators of epithelial to messenchymal transition and migration assay. Results: Our findings demonstrated for the first time the clinical correlation of plasma OPN with metastasis of CRC patients. High post-operative plasma OPN level (>153.02 ng/ml) associated with development of metastasis after curative resection (p<0.001). Moreover, postoperative plasma OPN level correlated with disease-free survival of CRC patients (p=0.009) and was an independent factor for predicting development of metastasis in CRC patients after curative resection (p=0.036). Our in vitro model showed that OPN ectopic expression induced DLD1 cell migration through Snail and Twist1 overexpression and E-cadherin repression, and secretory OPN level enhanced cell migration. Conclusions: The results of the current study suggest that post-operative plasma OPN correlated with post-operative metastasis, suggesting that it is a potential non-invasive biomarker for the development of future metastasis in CRC patients. In addition, OPN was shown to be involved in the metastatic process and thus inhibition of OPN is a potential therapeutic approach to treat CRC patients.

Original languageEnglish
Article numbere0126219
JournalPLoS ONE
Issue number5
Publication statusPublished - 11 May 2015
Externally publishedYes


Dive into the research topics of 'Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer'. Together they form a unique fingerprint.

Cite this