TY - JOUR
T1 - pIMP-PH114 carrying blaIMP-4in a Klebsiella pneumoniae strain is closely related to other multidrug-resistant IncA/C2 plasmids
AU - Ho, Pak Leung
AU - Lo, Wai U.
AU - Chan, Jane
AU - Cheung, Yuk Yam
AU - Chow, Kin Hung
AU - Yam, Wing Cheong
AU - Lin, Chi Ho
AU - Que, Tak Lun
N1 - Funding Information:
This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Government of the HKSAR. We thank Pak-Chung Sham, Levina Lam, Ben Ho and Wilson Chan of the Centre for Genomic Science, University of Hong Kong for technical assistance. Part of the data was presented as an abstract at the 23rd European Society of Clinical Microbiology and Infectious Diseases (ECCMID) meeting, Berlin, Germany, 27–30 April 2013.
PY - 2014/2
Y1 - 2014/2
N2 - The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array blaIMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase blaDHA-1 and the mercury resistance operon, mer-RTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical blaIMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the blaIMP-4 arrays among different diverse groups of bacteria.
AB - The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array blaIMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase blaDHA-1 and the mercury resistance operon, mer-RTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical blaIMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the blaIMP-4 arrays among different diverse groups of bacteria.
UR - http://www.scopus.com/inward/record.url?scp=84898917945&partnerID=8YFLogxK
U2 - 10.1007/s00284-013-0471-x
DO - 10.1007/s00284-013-0471-x
M3 - Article
C2 - 24121549
AN - SCOPUS:84898917945
SN - 0343-8651
VL - 68
SP - 227
EP - 232
JO - Current Microbiology
JF - Current Microbiology
IS - 2
ER -