TY - JOUR
T1 - Phytodegradation of neonicotinoids in Cyperus papyrus from enzymatic and transcriptomic perspectives
AU - Liu, Huanping
AU - Tang, Xiaoyan
AU - Tam, Nora Fung yee
AU - Li, Qiwen
AU - Ruan, Weifeng
AU - Xu, Xiaomin
AU - Gao, Yanxia
AU - Yan, Qingyun
AU - Zhang, Xiaomeng
AU - Dai, Yunv
AU - Yang, Yang
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2024/1/15
Y1 - 2024/1/15
N2 - Neonicotinoids are widely used but environmentally hazardous insecticides. Constructed wetlands offer potential for neonicotinoid removal, but the corresponding metabolic pathways and mechanisms in wetland plants are incompletely understood. This study investigated the fate of six neonicotinoids and their metabolites in Cyperus papyrus, a common wetland plant, and the underlying metabolic mechanisms through enzymatic and transcriptomic analyses. Neonicotinoids were absorbed by roots and translocated upward, causing high levels in shoots. Concentrations of neonicotinoids and their metabolites declined to their minimum at day 28 of exposure. Nitro reduction, hydroxylation, and demethylation were the major metabolic reactions with which C. papyrus responded to neonicotinoids. These reactions may be mediated by cytochrome P450 enzyme, aldehyde oxidase, glutathione-disulfide reductase, and glucuronate reductase. The toxicity of neonicotinoids in C. papyrus was evaluated according to the peroxidase and catalase enzymatic activities. Transcriptomic analysis revealed that differentially expressed genes (DEGs) mainly encoded proteins related to immune processes and cell growth regulation. Co-expression correlation analysis of DEGs revealed that the genes encoding P450s, peroxidase and glutathione S-transferase were the key functional genes. This study elucidates the stress response and degradation mechanism of neonicotinoids in wetland plants, providing new insights into the phytoremediation of organic contaminants in constructed wetlands.
AB - Neonicotinoids are widely used but environmentally hazardous insecticides. Constructed wetlands offer potential for neonicotinoid removal, but the corresponding metabolic pathways and mechanisms in wetland plants are incompletely understood. This study investigated the fate of six neonicotinoids and their metabolites in Cyperus papyrus, a common wetland plant, and the underlying metabolic mechanisms through enzymatic and transcriptomic analyses. Neonicotinoids were absorbed by roots and translocated upward, causing high levels in shoots. Concentrations of neonicotinoids and their metabolites declined to their minimum at day 28 of exposure. Nitro reduction, hydroxylation, and demethylation were the major metabolic reactions with which C. papyrus responded to neonicotinoids. These reactions may be mediated by cytochrome P450 enzyme, aldehyde oxidase, glutathione-disulfide reductase, and glucuronate reductase. The toxicity of neonicotinoids in C. papyrus was evaluated according to the peroxidase and catalase enzymatic activities. Transcriptomic analysis revealed that differentially expressed genes (DEGs) mainly encoded proteins related to immune processes and cell growth regulation. Co-expression correlation analysis of DEGs revealed that the genes encoding P450s, peroxidase and glutathione S-transferase were the key functional genes. This study elucidates the stress response and degradation mechanism of neonicotinoids in wetland plants, providing new insights into the phytoremediation of organic contaminants in constructed wetlands.
KW - Insecticide
KW - Key genes
KW - Metabolic pathway
KW - Phytodegradation
KW - Wetland plant
UR - http://www.scopus.com/inward/record.url?scp=85173812776&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2023.132715
DO - 10.1016/j.jhazmat.2023.132715
M3 - Article
C2 - 37844494
AN - SCOPUS:85173812776
SN - 0304-3894
VL - 462
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 132715
ER -