TY - JOUR
T1 - One-step extraction and derivatization liquid-phase microextraction for the determination of chlorophenols by gas chromatography-mass spectrometry
AU - Wang, Xiaowei
AU - Luo, Lijuan
AU - Ouyang, Gangfeng
AU - Lin, Li
AU - Tam, Nora F.Y.
AU - Lan, Chongyu
AU - Luan, Tiangang
N1 - Funding Information:
The financial support from National Natural Science Foundation of China (NSFC, no. U0633002, no. 40672212) and 863 Hi-Tech Research and Development Program of China (no. 2004AA649130) as well as Research Grant Council of the Hong Kong SAR (Project No. RGC Ref: CityU 1110/02 M) are gratefully acknowledged.
PY - 2009/8/28
Y1 - 2009/8/28
N2 - A sample pretreatment method for the determination of 18 chlorophenols (CPs) in aqueous samples by derivatization liquid-phase microextraction (LPME) was investigated using gas chromatography-mass spectrometry. Derivatization reagent was spiked into the extraction solvent to combine derivatization and extraction into one step. High sensitivity of 18 CPs derivatives could be achieved after optimization of several parameters such as extraction solvent, percentage of derivatization reagent, extraction time, pH, and ionic strength. The results from the optimal method showed that calibration ranging from 0.5 to 500 μg L-1 could be achieved with the RSDs between 1.75% and 9.39%, and the limits of detection (LOD) are ranging from 0.01 to 0.12 μg L-1 for the CPs. Moreover, the proposed LPME method was compared with solid-phase microextraction (SPME) coupled with on-fiber derivatization technique. The results suggested that using both methods are quite agreeable. Furthermore, the recoveries of LPME evaluated by spiked environmental samples ranged from 87.9% (3,5-DCP) to 114.7% (2,3,5,6-TeCP), and environmental water samples collected from the Pearl River were analyzed with the optimized LPME method, the concentrations of 18 CPs ranged from 0.0237 μg L-1 (3,5-DCP) to 0.3623 μg L-1 (2,3,6-TCP).
AB - A sample pretreatment method for the determination of 18 chlorophenols (CPs) in aqueous samples by derivatization liquid-phase microextraction (LPME) was investigated using gas chromatography-mass spectrometry. Derivatization reagent was spiked into the extraction solvent to combine derivatization and extraction into one step. High sensitivity of 18 CPs derivatives could be achieved after optimization of several parameters such as extraction solvent, percentage of derivatization reagent, extraction time, pH, and ionic strength. The results from the optimal method showed that calibration ranging from 0.5 to 500 μg L-1 could be achieved with the RSDs between 1.75% and 9.39%, and the limits of detection (LOD) are ranging from 0.01 to 0.12 μg L-1 for the CPs. Moreover, the proposed LPME method was compared with solid-phase microextraction (SPME) coupled with on-fiber derivatization technique. The results suggested that using both methods are quite agreeable. Furthermore, the recoveries of LPME evaluated by spiked environmental samples ranged from 87.9% (3,5-DCP) to 114.7% (2,3,5,6-TeCP), and environmental water samples collected from the Pearl River were analyzed with the optimized LPME method, the concentrations of 18 CPs ranged from 0.0237 μg L-1 (3,5-DCP) to 0.3623 μg L-1 (2,3,6-TCP).
KW - Chlorophenols
KW - Derivatization
KW - Gas chromatography-mass spectrometry (GC-MS)
KW - Liquid-phase microextraction
UR - http://www.scopus.com/inward/record.url?scp=67849105113&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2009.07.011
DO - 10.1016/j.chroma.2009.07.011
M3 - Article
C2 - 19640544
AN - SCOPUS:67849105113
SN - 0021-9673
VL - 1216
SP - 6267
EP - 6273
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 35
ER -