@inbook{f1f5af7731c44fa288ddb40f45c97f30,
title = "Methods to study the effects of TRP channel drugs on vascular endothelial cell function",
abstract = "Transient receptor potential (TRP) channels are abundantly expressed in vascular endothelium where the channels play important functional roles. In this chapter, we describe some well-established approaches, from molecular detection to functional assays, to study the role of TRP channels in endothelial cells. Comprehensive step-by-step instructions and representative figures are provided on the following methods: (1) isolation and culture of vascular endothelial cells; (2) determination of the TRP channel interaction [co-immunoprecipitation, double immunofluorescence staining, chemical cross-linking, and F{\"o}rster resonance energy transfer (FRET) detection]; (3) detection of TRP channel-mediated intracellular Ca2+ changes using fluorescence microscopy; (4) determination of the electrophysiological properties by whole-cell patch clamp and examination of TRP channel function in vascular tone control using isometric tension measurement.",
keywords = "Electrophysiology, Protein interaction, TRP channels, Vascular function",
author = "Yan Ma and Tjong, {Yung Wui} and Xiaoqiang Yao",
year = "2012",
doi = "10.1007/978-1-62703-095-3_3",
language = "English",
isbn = "9781627030946",
series = "Methods in Pharmacology and Toxicology",
pages = "55--74",
booktitle = "TRP Channels in Drug Discovery",
}