Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Maoyong Song; Yan Xu; Qinting Jiang; Paul K.S. Lam; Desmond K. O'Toole; John P. Giesy; Guibin Jiang

doi:10.1016/j.envint.2006.03.002

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Maoyong Song
, Yan Xu
, Qinting Jiang
, Paul K.S. Lam
, Desmond K. O'Toole
, John P. Giesy
, Guibin Jiang

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g^{- 1} dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction 2 > Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA.

Original language	English
Pages (from-to)	676-681
Number of pages	6
Journal	Environment International
Volume	32
Issue number	5
DOIs	https://doi.org/10.1016/j.envint.2006.03.002
Publication status	Published - Jul 2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

E2-EQs
Estrogenic activity
Haihe and Dagu Rivers
In vitro bioassay
MCF-7 cell line
Sediments

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10.1016/j.envint.2006.03.002

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@article{a66f3e9e4faa4cfc8e41f7aad23d1806,

title = "Measurement of estrogenic activity in sediments from Haihe and Dagu River, China",

abstract = "Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g- 1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction 2 > Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA.",

keywords = "E2-EQs, Estrogenic activity, Haihe and Dagu Rivers, In vitro bioassay, MCF-7 cell line, Sediments",

author = "Maoyong Song and Yan Xu and Qinting Jiang and Lam, \{Paul K.S.\} and O'Toole, \{Desmond K.\} and Giesy, \{John P.\} and Guibin Jiang",

note = "Funding Information: This study was supported by a Central Allocation Grant (8730020) awarded by the Research Grants Council, Hong Kong, and the Area of Excellence Scheme under the University Grants Committee of the Hong Kong Special Administration Region, China (Project No. AoE/P-04/2004). ",

year = "2006",

month = jul,

doi = "10.1016/j.envint.2006.03.002",

language = "English",

volume = "32",

pages = "676--681",

number = "5",

}

TY - JOUR

T1 - Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

AU - Song, Maoyong

AU - Xu, Yan

AU - Jiang, Qinting

AU - Lam, Paul K.S.

AU - O'Toole, Desmond K.

AU - Giesy, John P.

AU - Jiang, Guibin

N1 - Funding Information: This study was supported by a Central Allocation Grant (8730020) awarded by the Research Grants Council, Hong Kong, and the Area of Excellence Scheme under the University Grants Committee of the Hong Kong Special Administration Region, China (Project No. AoE/P-04/2004).

PY - 2006/7

Y1 - 2006/7

N2 - Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g- 1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction 2 > Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA.

AB - Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g- 1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction 2 > Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA.

KW - E2-EQs

KW - Estrogenic activity

KW - Haihe and Dagu Rivers

KW - In vitro bioassay

KW - MCF-7 cell line

KW - Sediments

UR - https://www.scopus.com/pages/publications/33646808083

U2 - 10.1016/j.envint.2006.03.002

DO - 10.1016/j.envint.2006.03.002

M3 - Article

C2 - 16624408

AN - SCOPUS:33646808083

SN - 0160-4120

VL - 32

SP - 676

EP - 681

JO - Environment International

JF - Environment International

IS - 5

ER -

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Abstract

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Keywords

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