@inbook{5a48785006fb44e095dc6db59135111a,
title = "Live imaging of planaria",
abstract = "Planarian regeneration involves a complex series of cellular events, precisely choreographed in space and time. Time-lapse imaging can provide powerful insights into tissue dynamics, as variously demonstrated in other model systems. However, time-lapse imaging of planarians has proven to be a challenge. Especially the requisite immobilization of the animals over extended periods of time is difficult, owing to their photophobic behavior and soft body architecture. Here, we describe a new embedding method using 2\% (w/v) low melting agarose, and demonstrate that this method can effectively immobilize animals as long as 7 days. In combination with cell-permeable fluorescent dyes, this immobilization method allows for the time-lapse imaging of planaria during regeneration and other physiological processes.",
keywords = "Fluorescent dye labeling, Live imaging, Planarian, Regeneration, Time-lapse microscopy",
author = "Wei Shen and Yun Shen and Lam, \{Yun Wah\} and Danny Chan",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7802-1\_22",
language = "English",
series = "Methods in Molecular Biology",
pages = "507--518",
booktitle = "Methods in Molecular Biology",
}