TY - JOUR
T1 - Interleukin-4 increases phagocytosis of necrotic cells by macrophages through scavenger receptor CD36
AU - Chen, Mingzhuang
AU - Tse, Gary
AU - Wong, Wing Tak
N1 - Publisher Copyright:
© 2020 John Wiley & Sons Australia, Ltd
PY - 2021/1
Y1 - 2021/1
N2 - Objective: Interleukin-4 (IL-4) signalling pathways regulate the activity of macrophages, enhance their proteolytic capacity and drive resolution of inflammation during tissue repair. The aim of this study was to examine whether IL-4 can enhance phagocytosis of necrotic cells and elucidate the molecular mechanisms. Methods: Phagocytosis of necrotic thymocytes by RAW264.7 cells, a macrophage cell line, with or without IL-4 treatment, was determined by flow cytometry. Protein expression was determined by western blot analysis. Results: The phagocytosis index was significantly increased by IL-4 (10 ng/mL). IL-4-enhanced phagocytosis was mediated by upregulation of scavenger receptor CD36. STAT6 activation is required for IL-4-mediated phagocytosis. Conclusions: Interleukin-4 can accelerate cell debris clearance by stimulating expression of CD36, which requires downstream STAT6 activation. Its beneficial effects on driving tissue repair and regeneration should be explored in future studies.
AB - Objective: Interleukin-4 (IL-4) signalling pathways regulate the activity of macrophages, enhance their proteolytic capacity and drive resolution of inflammation during tissue repair. The aim of this study was to examine whether IL-4 can enhance phagocytosis of necrotic cells and elucidate the molecular mechanisms. Methods: Phagocytosis of necrotic thymocytes by RAW264.7 cells, a macrophage cell line, with or without IL-4 treatment, was determined by flow cytometry. Protein expression was determined by western blot analysis. Results: The phagocytosis index was significantly increased by IL-4 (10 ng/mL). IL-4-enhanced phagocytosis was mediated by upregulation of scavenger receptor CD36. STAT6 activation is required for IL-4-mediated phagocytosis. Conclusions: Interleukin-4 can accelerate cell debris clearance by stimulating expression of CD36, which requires downstream STAT6 activation. Its beneficial effects on driving tissue repair and regeneration should be explored in future studies.
KW - CD36
KW - IL-4
KW - STAT6
KW - necrotic cells
KW - phagocytosis
UR - http://www.scopus.com/inward/record.url?scp=85091033979&partnerID=8YFLogxK
U2 - 10.1111/1440-1681.13399
DO - 10.1111/1440-1681.13399
M3 - Article
C2 - 32852093
AN - SCOPUS:85091033979
SN - 0305-1870
VL - 48
SP - 129
EP - 136
JO - Clinical and Experimental Pharmacology and Physiology
JF - Clinical and Experimental Pharmacology and Physiology
IS - 1
ER -