TY - JOUR
T1 - Highly sensitive and cost-effective portable sensor for early gastric carcinoma diagnosis
AU - Oo, Saw Lin
AU - Venkatesh, Shishir
AU - Karthikeyan, Vaithinathan
AU - Arava, Clement Manohar
AU - Pathikonda, Spoorthy
AU - Yu, Peter K.N.
AU - Lau, Terrence C.K.
AU - Chen, Xianfeng
AU - Roy, Vellaisamy A.L.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4/2
Y1 - 2021/4/2
N2 - Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1 mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.
AB - Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1 mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.
KW - Cancer antigen
KW - Cancer early detection
KW - Electrical current sensor
KW - Oligo RNA
KW - Polycarbonate membrane
UR - http://www.scopus.com/inward/record.url?scp=85103825846&partnerID=8YFLogxK
U2 - 10.3390/s21082639
DO - 10.3390/s21082639
M3 - Article
C2 - 33918707
AN - SCOPUS:85103825846
SN - 1424-8220
VL - 21
JO - Sensors
JF - Sensors
IS - 8
M1 - 2639
ER -