Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin

Xiaoyun Shen; P. K.S. Lam; G. R. Shaw; W. Wickramasinghe

doi:10.1016/S0041-0101(02)00151-4

Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin

Xiaoyun Shen, P. K.S. Lam, G. R. Shaw, W. Wickramasinghe

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity.

Original language	English
Pages (from-to)	1499-1501
Number of pages	3
Journal	Toxicon
Volume	40
Issue number	10
DOIs	https://doi.org/10.1016/S0041-0101(02)00151-4
Publication status	Published - 1 Oct 2002
Externally published	Yes

Keywords

Alkaline gel electrophoresis
Cylindrospermopsin
DNA strand breaks
Genotoxicity

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10.1016/S0041-0101(02)00151-4

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title = "Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin",

abstract = "Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity.",

keywords = "Alkaline gel electrophoresis, Cylindrospermopsin, DNA strand breaks, Genotoxicity",

author = "Xiaoyun Shen and Lam, {P. K.S.} and Shaw, {G. R.} and W. Wickramasinghe",

note = "Funding Information: This project was funded by a strategic research grant of the City University of Hong Kong (7001282).",

year = "2002",

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doi = "10.1016/S0041-0101(02)00151-4",

language = "English",

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T1 - Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin

AU - Shen, Xiaoyun

AU - Lam, P. K.S.

AU - Shaw, G. R.

AU - Wickramasinghe, W.

N1 - Funding Information: This project was funded by a strategic research grant of the City University of Hong Kong (7001282).

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity.

AB - Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity.

KW - Alkaline gel electrophoresis

KW - Cylindrospermopsin

KW - DNA strand breaks

KW - Genotoxicity

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U2 - 10.1016/S0041-0101(02)00151-4

DO - 10.1016/S0041-0101(02)00151-4

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C2 - 12368121

AN - SCOPUS:0036802862

SN - 0041-0101

VL - 40

SP - 1499

EP - 1501

JO - Toxicon

JF - Toxicon

IS - 10

ER -

Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin

Abstract

Keywords

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