TY - JOUR
T1 - Gating a Single Cell
T2 - A Label-Free and Real-Time Measurement Method for Cellular Progression
AU - Oo, Saw Lin
AU - Venkatesh, Shishir
AU - Ilyas, Abdul Mojeed
AU - Karthikeyan, Vaithinathan
AU - Arava, Clement Manohar
AU - Kong, Eva Yi
AU - Yeung, Chi Chung
AU - Chen, Xianfeng
AU - Yu, Peter K.N.
AU - Roy, Vellaisamy A.L.
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/1/21
Y1 - 2020/1/21
N2 - There is an ever-growing need for more advanced methods to study the response of cancer cells to new therapies. To determine cancer cells' response from a cell-mortality perspective to various cancer therapies, we report a label-free and real time method to monitor the in situ response of individual HeLa cells using a single cell gated transistor (SCGT). As a cell undergoes apoptotic cell death, it experiences changes in morphology and ion concentrations. This change is well in line with the threshold voltage of the SCGT, which has been verified by correlating the data with the cell morphologies by scanning electron microscopy and the ion-concentration analysis by inductively-coupled plasma mass spectrometry (ICPMS). This SCGT could replace patch clamps to study single cell activity via direct measurement in real time. Importantly, this SCGT can be used to study the electrical response of a single cell to stimuli that leaves the membrane intact.
AB - There is an ever-growing need for more advanced methods to study the response of cancer cells to new therapies. To determine cancer cells' response from a cell-mortality perspective to various cancer therapies, we report a label-free and real time method to monitor the in situ response of individual HeLa cells using a single cell gated transistor (SCGT). As a cell undergoes apoptotic cell death, it experiences changes in morphology and ion concentrations. This change is well in line with the threshold voltage of the SCGT, which has been verified by correlating the data with the cell morphologies by scanning electron microscopy and the ion-concentration analysis by inductively-coupled plasma mass spectrometry (ICPMS). This SCGT could replace patch clamps to study single cell activity via direct measurement in real time. Importantly, this SCGT can be used to study the electrical response of a single cell to stimuli that leaves the membrane intact.
UR - https://www.scopus.com/pages/publications/85078370114
U2 - 10.1021/acs.analchem.9b03136
DO - 10.1021/acs.analchem.9b03136
M3 - Article
C2 - 31904934
AN - SCOPUS:85078370114
SN - 0003-2700
VL - 92
SP - 1738
EP - 1745
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 2
ER -
