Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

C. K.M. Ng; E. Y.W. Chong; V. A.L. Roy; K. M.C. Cheung; K. W.K. Yeung; K. N. Yu

doi:10.1016/j.apradiso.2011.11.019

Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

C. K.M. Ng
, E. Y.W. Chong
, V. A.L. Roy
, K. M.C. Cheung
, K. W.K. Yeung
, K. N. Yu

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.

Original language	English
Pages (from-to)	1432-1435
Number of pages	4
Journal	Applied Radiation and Isotopes
Volume	70
Issue number	7
DOIs	https://doi.org/10.1016/j.apradiso.2011.11.019
Publication status	Published - Jul 2012
Externally published	Yes

Keywords

Alpha particle
Cell proliferation
HeLa cells
Micropillar substrate
PADC
PDMS

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10.1016/j.apradiso.2011.11.019

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title = "Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films",

abstract = "We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.",

keywords = "Alpha particle, Cell proliferation, HeLa cells, Micropillar substrate, PADC, PDMS",

author = "Ng, \{C. K.M.\} and Chong, \{E. Y.W.\} and Roy, \{V. A.L.\} and Cheung, \{K. M.C.\} and Yeung, \{K. W.K.\} and Yu, \{K. N.\}",

year = "2012",

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doi = "10.1016/j.apradiso.2011.11.019",

language = "English",

volume = "70",

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TY - JOUR

T1 - Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

AU - Ng, C. K.M.

AU - Chong, E. Y.W.

AU - Roy, V. A.L.

AU - Cheung, K. M.C.

AU - Yeung, K. W.K.

AU - Yu, K. N.

PY - 2012/7

Y1 - 2012/7

N2 - We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.

AB - We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.

KW - Alpha particle

KW - Cell proliferation

KW - HeLa cells

KW - Micropillar substrate

KW - PADC

KW - PDMS

UR - https://www.scopus.com/pages/publications/84862155947

U2 - 10.1016/j.apradiso.2011.11.019

DO - 10.1016/j.apradiso.2011.11.019

M3 - Article

AN - SCOPUS:84862155947

SN - 0969-8043

VL - 70

SP - 1432

EP - 1435

JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

IS - 7

ER -

Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

Abstract

Keywords

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