TY - JOUR
T1 - Effects of nutrients, salinity, pH and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina
AU - Liu, Wenhua
AU - Au, Doris W.T.
AU - Anderson, Donald M.
AU - Lam, Paul K.S.
AU - Wu, Rudolf S.S.
N1 - Funding Information:
We thank Prof. Watanabe Masataka of the National Institute of Environmental Studies (NIES), Japan for providing a stock of C. marina culture. The work described in this paper was supported by a CERG grant and an AoE grant from the University Grants Committee of the Hong Kong Special Administrative Region, China to RSSW (Projects No. 9040864 and AoE/P-04/2004)). Support for DMA is provided by U.S. National Science Foundation grant # OCE-0136861. [SS]
PY - 2007/6/15
Y1 - 2007/6/15
N2 - Experiments were carried out to investigate the effects of nutrients, salinity, pH and light:dark cycle on growth rate and production of reactive oxygen species (ROS) by Chattonella marina, a harmful algal bloom (HAB) species that often causes fish kills. Different nitrogen forms (organic-N and inorganic-N), N:P ratios, light:dark cycles and salinity significantly influenced algal growth, but not ROS production. However, iron concentration and pH significantly affected both growth and ROS production in C. marina. KCN (an inhibitor of mitochondrial respiration) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (an inhibitor of photosynthesis) had no significant effects on ROS production. Vitamin K3 (a plasma membrane electron shuttle) enhanced ROS production while its antagonist, dicumarol, decreased ROS production. Taken together, our results suggest that ROS production by C. marina is related to a plasma membrane enzyme system regulated by iron availability but is independent of growth, photosynthesis, availability of macronutrients, salinity and irradiance.
AB - Experiments were carried out to investigate the effects of nutrients, salinity, pH and light:dark cycle on growth rate and production of reactive oxygen species (ROS) by Chattonella marina, a harmful algal bloom (HAB) species that often causes fish kills. Different nitrogen forms (organic-N and inorganic-N), N:P ratios, light:dark cycles and salinity significantly influenced algal growth, but not ROS production. However, iron concentration and pH significantly affected both growth and ROS production in C. marina. KCN (an inhibitor of mitochondrial respiration) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (an inhibitor of photosynthesis) had no significant effects on ROS production. Vitamin K3 (a plasma membrane electron shuttle) enhanced ROS production while its antagonist, dicumarol, decreased ROS production. Taken together, our results suggest that ROS production by C. marina is related to a plasma membrane enzyme system regulated by iron availability but is independent of growth, photosynthesis, availability of macronutrients, salinity and irradiance.
KW - Chattonella marina
KW - Iron
KW - Nutrient
KW - Physical parameters
KW - Plasma membrane
KW - Reactive oxygen species
KW - Redox
KW - pH
UR - http://www.scopus.com/inward/record.url?scp=34248155180&partnerID=8YFLogxK
U2 - 10.1016/j.jembe.2007.03.007
DO - 10.1016/j.jembe.2007.03.007
M3 - Article
AN - SCOPUS:34248155180
SN - 0022-0981
VL - 346
SP - 76
EP - 86
JO - Journal of Experimental Marine Biology and Ecology
JF - Journal of Experimental Marine Biology and Ecology
IS - 1-2
ER -