TY - JOUR
T1 - Differential expression of filamin B splice variants in giant cell tumor cells
AU - Tsui, Joseph Chi Ching
AU - Lau, Carol Po Ying
AU - Cheung, Alex Chun
AU - Wong, Kwok Chuen
AU - Huang, Lin
AU - Tsui, Stephen Kwok Wing
AU - Kumta, Shekhar Madhukar
N1 - Funding Information:
This study is supported by the Health and Medical Research Fund from the Food and Health Bureau, Hong Kong Special Administrative Region (reference no. 14130312).
PY - 2016/12
Y1 - 2016/12
N2 - Giant cell tumor of bone (GCT) is the most commonly reported non-malignant bone tumor in Hong Kong. This kind of tumor usually affects people aged 20-40 years. Also, it is well known for recurrence locally, especially when the tumor cannot be removed completely. Filamins are actin-binding proteins which contain three family members, filamin A, B and C. They are the products of three different genes, FLNA, FLNB and FLNC, which can generate various transcript variants in different cell types. In this study, we focused on the effects of FLNBv2 and FLNBv4 toward GCT cells. The only difference between FLNBv2 and FLNBv4 is that FLNBv4 does not contain hinge 1 region. We found that the relative abundance of FLNBv4 varies among different GCT cell lines while the expression level of FLNBv4 in normal osteoblasts was only marginally detectable. In the functional aspect, overexpression of FLNBv4 led to upregulation of RANKL, OCN, OPG and RUNX2, which are closely related to GCT cell survival and differentiation. Moreover, FLNBv4 can have a negative effect on cell viability of GCT cells when compare with FLNBv2. In conclusion, splicing variants of FLNB are differentially expressed in GCT cells and may play a role in the proliferation and differentiation of tumor cells.
AB - Giant cell tumor of bone (GCT) is the most commonly reported non-malignant bone tumor in Hong Kong. This kind of tumor usually affects people aged 20-40 years. Also, it is well known for recurrence locally, especially when the tumor cannot be removed completely. Filamins are actin-binding proteins which contain three family members, filamin A, B and C. They are the products of three different genes, FLNA, FLNB and FLNC, which can generate various transcript variants in different cell types. In this study, we focused on the effects of FLNBv2 and FLNBv4 toward GCT cells. The only difference between FLNBv2 and FLNBv4 is that FLNBv4 does not contain hinge 1 region. We found that the relative abundance of FLNBv4 varies among different GCT cell lines while the expression level of FLNBv4 in normal osteoblasts was only marginally detectable. In the functional aspect, overexpression of FLNBv4 led to upregulation of RANKL, OCN, OPG and RUNX2, which are closely related to GCT cell survival and differentiation. Moreover, FLNBv4 can have a negative effect on cell viability of GCT cells when compare with FLNBv2. In conclusion, splicing variants of FLNB are differentially expressed in GCT cells and may play a role in the proliferation and differentiation of tumor cells.
KW - Filamin B
KW - Giant cell tumor of bone
KW - Splicing variants
UR - http://www.scopus.com/inward/record.url?scp=84994665987&partnerID=8YFLogxK
U2 - 10.3892/or.2016.5197
DO - 10.3892/or.2016.5197
M3 - Article
C2 - 27779699
AN - SCOPUS:84994665987
SN - 1021-335X
VL - 36
SP - 3181
EP - 3187
JO - Oncology Reports
JF - Oncology Reports
IS - 6
ER -
