TY - JOUR
T1 - Development of a carboxyl-terminated indium tin oxide electrode for improving cell adhesion and facilitating low noise, real-time impedance measurements
AU - Ilyas, A. M.Olabisi
AU - Alam, Md Kowsar
AU - Musah, Jamal Deen
AU - Saw, Lin Oo
AU - Venkatesh, Shishir
AU - Yeung, Chi Chung
AU - Yang, Mengsu
AU - Vellaisamy, A. L.R.
AU - Lau, Condon
N1 - Publisher Copyright:
© 2021 American Physiological Society. All rights reserved.
PY - 2021/6
Y1 - 2021/6
N2 - The working electrode's surface property is crucial to cell adhesion and signal collection in electric cell-substrate impedance sensing (ECIS). To date, the indium tin oxide (ITO)-based working electrode is of interest in ECIS study due to its high transparency and biocompatibility. Of great concern is the impedance signal loss, distortion, and data interpretation conflict profoundly created by the movement of multiple cells during ECIS study. Here, a carboxyl-terminated ITO substrate was prepared by stepwise surface amino silanization, with N-hydroxy succinimide (NHS) and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) treatment, respectively. We investigated the stepwise changes in the property of the treated ITO, cell-substrate adhesion, collective cell mobility, and time course of change in absolute impedance from multiple Chinese hamster ovary (CHO) cells [(Dt-DjZj)CELLS]. The carboxyl-terminated ITO substrate with a surface roughness of 6.37nm shows enhanced conductivity, 75% visible light transparency, improved cell adherence, reduced collective cell migration speed by approximately twofold, and diminished signal distortion in the [(Dt-DjZj)CELLS]. Thus, our study provides an ITO surface-treatment strategy to reduce multiple cell movement effects and to obtain essential cell information from the ECIS study of multiple cells through undistorted (Dt-DjZj)CELLS.
AB - The working electrode's surface property is crucial to cell adhesion and signal collection in electric cell-substrate impedance sensing (ECIS). To date, the indium tin oxide (ITO)-based working electrode is of interest in ECIS study due to its high transparency and biocompatibility. Of great concern is the impedance signal loss, distortion, and data interpretation conflict profoundly created by the movement of multiple cells during ECIS study. Here, a carboxyl-terminated ITO substrate was prepared by stepwise surface amino silanization, with N-hydroxy succinimide (NHS) and 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) treatment, respectively. We investigated the stepwise changes in the property of the treated ITO, cell-substrate adhesion, collective cell mobility, and time course of change in absolute impedance from multiple Chinese hamster ovary (CHO) cells [(Dt-DjZj)CELLS]. The carboxyl-terminated ITO substrate with a surface roughness of 6.37nm shows enhanced conductivity, 75% visible light transparency, improved cell adherence, reduced collective cell migration speed by approximately twofold, and diminished signal distortion in the [(Dt-DjZj)CELLS]. Thus, our study provides an ITO surface-treatment strategy to reduce multiple cell movement effects and to obtain essential cell information from the ECIS study of multiple cells through undistorted (Dt-DjZj)CELLS.
KW - Cell adherence
KW - Cell mobility
KW - Cho cells
KW - Impedance spectroscopy
KW - Working electrode
UR - http://www.scopus.com/inward/record.url?scp=85107711379&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.00537.2020
DO - 10.1152/ajpcell.00537.2020
M3 - Article
C2 - 33689477
AN - SCOPUS:85107711379
SN - 0363-6143
VL - 320
SP - C974-C986
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 6
ER -