Determination of polychlorinated biphenyls in human blood serum by SPME

Ka Fai Poon; Paul K.S. Lam; Michael H.W. Lam

doi:10.1016/S0045-6535(99)00033-8

Determination of polychlorinated biphenyls in human blood serum by SPME

Ka Fai Poon
, Paul K.S. Lam
, Michael H.W. Lam

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We report a direct immersion SPME - GC/μECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved.

Original language	English
Pages (from-to)	905-912
Number of pages	8
Journal	Chemosphere
Volume	39
Issue number	6
DOIs	https://doi.org/10.1016/S0045-6535(99)00033-8
Publication status	Published - Sept 1999
Externally published	Yes

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10.1016/S0045-6535(99)00033-8

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title = "Determination of polychlorinated biphenyls in human blood serum by SPME",

abstract = "We report a direct immersion SPME - GC/μECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9\% to 3.4\% (n = 10) was obtained. Analyte recovery up to 93\% and detection limit of 1.0 ppb (total PCB) were achieved.",

author = "Poon, \{Ka Fai\} and Lam, \{Paul K.S.\} and Lam, \{Michael H.W.\}",

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AU - Poon, Ka Fai

AU - Lam, Paul K.S.

AU - Lam, Michael H.W.

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Y1 - 1999/9

N2 - We report a direct immersion SPME - GC/μECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved.

AB - We report a direct immersion SPME - GC/μECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved.

UR - https://www.scopus.com/pages/publications/0033200850

U2 - 10.1016/S0045-6535(99)00033-8

DO - 10.1016/S0045-6535(99)00033-8

M3 - Article

C2 - 10448565

AN - SCOPUS:0033200850

SN - 0045-6535

VL - 39

SP - 905

EP - 912

JO - Chemosphere

JF - Chemosphere

IS - 6

ER -

Determination of polychlorinated biphenyls in human blood serum by SPME

Abstract

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