Determination of hydroxy metabolites of polycyclic aromatic hydrocarbons by fully automated solid-phase microextraction derivatization and gas chromatography-mass spectrometry

Tiangang Luan, Shenghong Fang, Yin Zhong, Li Lin, Sidney M.N. Chan, Chongyu Lan, Nora F.Y. Tam

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

A fully automated sample pretreatment method was developed for the detection of mono and dihydroxy metabolites of polycyclic aromatic hydrocarbons (PAHs) by gas chromatography-mass spectrometry in the selected ion monitoring mode. Direct immersion solid-phase microextraction for the extraction of target compounds and the headspace on-fiber silylation with N,O-bis(trimethylsilyl)trifluoroacetamide were performed automatically by a multipurpose autosampler (MPS2). The operating conditions including extraction time, derivatization time, ionic strength, pH, and incubation temperature were optimized. Calibration responses of nine metabolites of PAHs over a concentration range of 0.1-100 μg L-1 with a correlation coefficient of 0.999 were obtained. The detection limits of the nine metabolites in mini pore water, minimal salts medium and soil extract culture medium were in the range of 0.001-0.013, 0.002-0.024 and 0.002-0.134 μg L-1, respectively, while the respective quantification limits were 0.003-0.044, 0.005-0.081 and 0.008-0.447 μg L-1. The reliability was confirmed by the traditional solid-phase extraction method. The proposed method could be used to analyze the metabolites of PAHs degraded by microorganisms such as algae and to determine the biodegradation pathways of PAHs.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Chromatography A
Volume1173
Issue number1-2
DOIs
Publication statusPublished - 30 Nov 2007
Externally publishedYes

Keywords

  • Biodegradation
  • Metabolites
  • Microorganisms
  • Polycyclic aromatic hydrocarbons
  • Solid-phase microextraction (SPME)

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