TY - JOUR
T1 - Cultured gill epithelial cells from tilapia (Oreochromis niloticus)
T2 - A new in vitro assay for toxicants
AU - Zhou, Bingsheng
AU - Liu, Wenhua
AU - Wu, Rudolf S.S.
AU - Lam, Paul K.S.
N1 - Funding Information:
We would like to thank Dr. Chris Wood of McMaster University for sharing with us the fish gill cell culture technique developed in his laboratory and Dr. Scott Kelly, for teaching me (BSZ) the cell culture technique. Thanks are also due to the Agriculture, Fisheries and Conservation Department of Hong Kong SAR for providing the tilapia. We thank Michael Chiang for his excellent technical assistance with electron microscopy, and Dr. Graham Blackmore for reading the draft of this paper. The work described in this paper was supported by the Areas of Excellence Scheme established under the University Grants Committee of the Hong Kong Special Administrative Region, China (Project No. AoE/P-04/2004).
PY - 2005/1/18
Y1 - 2005/1/18
N2 - A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5- pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC 50 of EROD activity was 2.77 × 10 -7 M for PCB#126, 1.85 × 10 -7 M for B[a]P and 7.38 × 10 -10 M for TCDD, showing that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple, rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters.
AB - A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5- pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC 50 of EROD activity was 2.77 × 10 -7 M for PCB#126, 1.85 × 10 -7 M for B[a]P and 7.38 × 10 -10 M for TCDD, showing that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple, rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters.
KW - B[a]P
KW - EROD
KW - Gill epithelia
KW - PCB
KW - Primary cell culture
KW - TCDD
KW - Tilapia
UR - http://www.scopus.com/inward/record.url?scp=11844273193&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2004.10.013
DO - 10.1016/j.aquatox.2004.10.013
M3 - Article
C2 - 15642632
AN - SCOPUS:11844273193
SN - 0166-445X
VL - 71
SP - 61
EP - 72
JO - Aquatic Toxicology
JF - Aquatic Toxicology
IS - 1
ER -
