TY - JOUR
T1 - Comparative study on in vitro inhibition of grass carp (Ctenopharyngodon idellus) and mouse protein phosphatases by microcystins
AU - Xu, L.
AU - Lam, P. K.S.
AU - Chen, J.
AU - Zhang, Y.
AU - Harada, K.
PY - 2000
Y1 - 2000
N2 - Microcystins isolated from toxic cyanobacteria are potent inhibitors of protein phosphatases 1 and 2A (PP1 and PP2A). The inhibitory effects of three structural variants of microcystins (microcystin-LR, -YR, and -RR) on protein phosphatases isolated and purified from the liver and kidney of grass carp (Ctenopharyngodon idellus) were investigated using the 32P radiometric assay. The relationships between percentage inhibition of protein phosphatase activity and microcystin levels followed a typical dose-dependent sigmoid curve. These results were compared to those obtained from mouse PP2A. The degree and pattern of inhibition of both fish and mouse protein phosphatases by microcystins were similar. Protein phosphatases in crude fish tissue homogenates showed similar inhibition patterns as purified fish PP2A toward microcystins. (C) 2000 by John Wiley and Sons, Inc.
AB - Microcystins isolated from toxic cyanobacteria are potent inhibitors of protein phosphatases 1 and 2A (PP1 and PP2A). The inhibitory effects of three structural variants of microcystins (microcystin-LR, -YR, and -RR) on protein phosphatases isolated and purified from the liver and kidney of grass carp (Ctenopharyngodon idellus) were investigated using the 32P radiometric assay. The relationships between percentage inhibition of protein phosphatase activity and microcystin levels followed a typical dose-dependent sigmoid curve. These results were compared to those obtained from mouse PP2A. The degree and pattern of inhibition of both fish and mouse protein phosphatases by microcystins were similar. Protein phosphatases in crude fish tissue homogenates showed similar inhibition patterns as purified fish PP2A toward microcystins. (C) 2000 by John Wiley and Sons, Inc.
KW - Fish protein phosphatases
KW - In vitro inhibition
KW - Microcystins
UR - http://www.scopus.com/inward/record.url?scp=0034015738&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1522-7278(2000)15:2<71::AID-TOX1>3.0.CO;2-E
DO - 10.1002/(SICI)1522-7278(2000)15:2<71::AID-TOX1>3.0.CO;2-E
M3 - Article
AN - SCOPUS:0034015738
SN - 1520-4081
VL - 15
SP - 71
EP - 75
JO - Environmental Toxicology
JF - Environmental Toxicology
IS - 2
ER -