TY - JOUR
T1 - Combined toxicity of cadmium and copper in Avicennia marina seedlings and the regulation of exogenous jasmonic acid
AU - Yan, Zhongzheng
AU - Li, Xiuzhen
AU - Chen, Jun
AU - Tam, Nora Fung Yee
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Seedlings of Avicennia marina were exposed to single and combined metal treatments of cadmium (Cd) and copper (Cu) in a factorial design, and the combined toxicity of Cu and Cd was tested. The effects of the exogenous jasmonic acid (JA) on chlorophyll concentration, lipid peroxidation, Cd and Cu uptake, antioxidative capacity, endogenous JA concentration, and type-2 metallothionein gene (AmMT2) expression in seedlings of A. marina exposed to combined metal treatments were also investigated. A binary mixture of low-dose Cd (9μmolL-1) and high-dose Cu (900μmolL-1) showed toxicity to the seedlings, indicated by the significant augmentation in leaf malondialdehyde (MDA) and reduction in leaf chlorophylls. The toxicity of the combined metals was significantly alleviated by the addition of exogenous JA at 1μmolL-1, and the chlorophyll and MDA contents were found to be restored to levels comparable to those of the control. Compare to treatment with Cd and Cu only, 1 and 10μmolL-1 JA significantly enhanced the ascorbate peroxidase activity, and 10μmolL-1 JA significantly decreased the uptake of Cd in A. marina leaves. The relative expression of leaf AmMT2 gene was also significantly enhanced by 1 and 10μmolL-1 JA, which helped reduce Cd toxicity in A. marina seedlings.
AB - Seedlings of Avicennia marina were exposed to single and combined metal treatments of cadmium (Cd) and copper (Cu) in a factorial design, and the combined toxicity of Cu and Cd was tested. The effects of the exogenous jasmonic acid (JA) on chlorophyll concentration, lipid peroxidation, Cd and Cu uptake, antioxidative capacity, endogenous JA concentration, and type-2 metallothionein gene (AmMT2) expression in seedlings of A. marina exposed to combined metal treatments were also investigated. A binary mixture of low-dose Cd (9μmolL-1) and high-dose Cu (900μmolL-1) showed toxicity to the seedlings, indicated by the significant augmentation in leaf malondialdehyde (MDA) and reduction in leaf chlorophylls. The toxicity of the combined metals was significantly alleviated by the addition of exogenous JA at 1μmolL-1, and the chlorophyll and MDA contents were found to be restored to levels comparable to those of the control. Compare to treatment with Cd and Cu only, 1 and 10μmolL-1 JA significantly enhanced the ascorbate peroxidase activity, and 10μmolL-1 JA significantly decreased the uptake of Cd in A. marina leaves. The relative expression of leaf AmMT2 gene was also significantly enhanced by 1 and 10μmolL-1 JA, which helped reduce Cd toxicity in A. marina seedlings.
KW - Combined metals
KW - Jasmonic acid
KW - Mangrove
KW - Metallothioneins
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=84916213997&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2014.11.031
DO - 10.1016/j.ecoenv.2014.11.031
M3 - Article
C2 - 25497768
AN - SCOPUS:84916213997
SN - 0147-6513
VL - 113
SP - 124
EP - 132
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -