TY - JOUR
T1 - Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments
AU - Guo, Chuling
AU - Dang, Zhi
AU - Wong, Yukshan
AU - Tam, Nora Fungyee
N1 - Funding Information:
The research work described in this paper was supported by a grant from the Strategic Research Grant of the City University of Hong Kong (Project No.: 7008036), Natural Research Fund of Guangdong Province, Guangdong (Project No.: 9351064101000001) and the Fundamental Research Funds for the Central Universities of China (Project No.: 2009ZM0034).
PY - 2010
Y1 - 2010
N2 - The biodegradation ability and initial dioxygenase genes of 21 PAH-degrading bacteria isolated from mangrove sediments were investigated. Most of the isolates belonged to the genera of Sphingomonas and Mycobacterium, and the other included Rhodococcus, Paracoccus and Pseudomonas. All the isolated Mycobacterium strains could completely degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) comprising phenanthrene (Phe), fluoranthene (Fla) and pyrene (Pyr) in 14 days. On the other hand, the sphingomonads differed in the extent to which mixed PAHs were degraded from 3% to 79%. The co-culture of Sphingomonas and Mycobacterium strains enhanced the degradation and all three PAHs were completely removed in 7 days. Among the three PAHs, only Pyr was completely degraded by three Mycobacterium strains (SBSW, YOWG and SKEY), whereas Fla, and then Phe were degraded by these three and other isolates. The isolated Mycobacterium strains possessed the nidA gene encoding the initial dioxygenase required for Pyr degradation, while nahAc and phnAc were not detected in the sphingomonads.
AB - The biodegradation ability and initial dioxygenase genes of 21 PAH-degrading bacteria isolated from mangrove sediments were investigated. Most of the isolates belonged to the genera of Sphingomonas and Mycobacterium, and the other included Rhodococcus, Paracoccus and Pseudomonas. All the isolated Mycobacterium strains could completely degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) comprising phenanthrene (Phe), fluoranthene (Fla) and pyrene (Pyr) in 14 days. On the other hand, the sphingomonads differed in the extent to which mixed PAHs were degraded from 3% to 79%. The co-culture of Sphingomonas and Mycobacterium strains enhanced the degradation and all three PAHs were completely removed in 7 days. Among the three PAHs, only Pyr was completely degraded by three Mycobacterium strains (SBSW, YOWG and SKEY), whereas Fla, and then Phe were degraded by these three and other isolates. The isolated Mycobacterium strains possessed the nidA gene encoding the initial dioxygenase required for Pyr degradation, while nahAc and phnAc were not detected in the sphingomonads.
KW - Biodegradation
KW - Co-culture
KW - Dioxygenase gene
KW - Mycobacterium
KW - PAH-degrading bacteria
KW - Sphingomonads
UR - http://www.scopus.com/inward/record.url?scp=77954955759&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2010.04.008
DO - 10.1016/j.ibiod.2010.04.008
M3 - Article
AN - SCOPUS:77954955759
SN - 0964-8305
VL - 64
SP - 419
EP - 426
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 6
ER -