Alpha-particle radiobiological experiments using thin CR-39 detectors

K. F. Chan; S. Y.M. Siu; K. E. McClella; A. K.W. Tse; B. M.F. Lau; D. Nikezic; B. J. Richardson; P. K.S. Lam; W. F. Fong; K. N. Yu

doi:10.1093/rpd/ncl393

Alpha-particle radiobiological experiments using thin CR-39 detectors

K. F. Chan, S. Y.M. Siu, K. E. McClella, A. K.W. Tse, B. M.F. Lau, D. Nikezic, B. J. Richardson, P. K.S. Lam, W. F. Fong, K. N. Yu

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 μm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37°C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.

Original language	English
Pages (from-to)	160-162
Number of pages	3
Journal	Radiation Protection Dosimetry
Volume	122
Issue number	1-4
DOIs	https://doi.org/10.1093/rpd/ncl393
Publication status	Published - Dec 2006
Externally published	Yes

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title = "Alpha-particle radiobiological experiments using thin CR-39 detectors",

abstract = "The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 μm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37°C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.",

author = "Chan, {K. F.} and Siu, {S. Y.M.} and McClella, {K. E.} and Tse, {A. K.W.} and Lau, {B. M.F.} and D. Nikezic and Richardson, {B. J.} and Lam, {P. K.S.} and Fong, {W. F.} and Yu, {K. N.}",

year = "2006",

month = dec,

doi = "10.1093/rpd/ncl393",

language = "English",

volume = "122",

pages = "160--162",

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T1 - Alpha-particle radiobiological experiments using thin CR-39 detectors

AU - Chan, K. F.

AU - Siu, S. Y.M.

AU - McClella, K. E.

AU - Tse, A. K.W.

AU - Lau, B. M.F.

AU - Nikezic, D.

AU - Richardson, B. J.

AU - Lam, P. K.S.

AU - Fong, W. F.

AU - Yu, K. N.

PY - 2006/12

Y1 - 2006/12

N2 - The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 μm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37°C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.

AB - The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 μm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37°C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.

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DO - 10.1093/rpd/ncl393

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EP - 162

JO - Radiation Protection Dosimetry

JF - Radiation Protection Dosimetry

IS - 1-4

ER -

Alpha-particle radiobiological experiments using thin CR-39 detectors

Abstract

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