TY - JOUR
T1 - A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine
AU - Guo, Qiang
AU - Zhang, Jie
AU - Sun, Hongyan
AU - Chen, Jian Lin
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/6/15
Y1 - 2022/6/15
N2 - The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.
AB - The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.
KW - covalent bonding
KW - fluorescence quench
KW - graphene oxide
KW - hydrazine
KW - resorufin
UR - http://www.scopus.com/inward/record.url?scp=85129240684&partnerID=8YFLogxK
U2 - 10.1002/asia.202200060
DO - 10.1002/asia.202200060
M3 - Article
C2 - 35415962
AN - SCOPUS:85129240684
SN - 1861-4728
VL - 17
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
IS - 12
M1 - e202200060
ER -